Abstract
The soil archaeon Nitrosotalea devanaterra is a representative of an abundant ammonia oxidising archaeal lineage. The effects of three nitrification inhibitors (allylthiourea (ATU), dicyandiamide (DCD) and nitrapyrin) on growth in both soil and liquid culture were compared. DCD and nitrapyrin inhibited nitrification at similar concentrations to bacterial ammonia oxidisers. Although DCD completely inhibited nitrification, some growth occurred in liquid cultures. Surprisingly, Nitrosotalea devanaterra was less susceptible to inhibition by ATU, which stimulated, rather than inhibited, nitrification in soil microcosms. Inhibition thresholds and responses may reflect differences in ammonia oxidation mechanisms between archaea and bacteria.
Highlights
The soil archaeon Nitrosotalea devanaterra is a representative of an abundant ammonia oxidising archaeal lineage
Nitrosotalea devanaterra was less susceptible to inhibition by ATU, which stimulated, rather than inhibited, nitrification in soil microcosms
Ammonia oxidation by N. devanaterra was completely inhibited in liquid culture in the presence of 5 mM and 10 mM DCD (t-test, p 1⁄4 0.051 and p 1⁄4 0.51), while 1 mM DCD led to 33% (Æ1.7%, standard error) inhibition based on the final nitrite yield (t-test, p < 0.001) (Fig. 1a)
Summary
The effects of three nitrification inhibitors (allylthiourea (ATU), dicyandiamide (DCD) and nitrapyrin) on growth in both soil and liquid culture were compared. The effects of three nitrification inhibitors, DCD, ATU and nitrapyrin, were investigated by monitoring growth (amoA gene abundance) and activity (process rates) of N. devanaterra in liquid batch culture and in soil microcosms.
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