Abstract
Amino acids present in blood plasma may be targets for oxidation and chlorination by HOCl/OCl−. N-Chloroamino acids have been reported to be less reactive, but more selective than HOCl/OCl− in their reactions; therefore, they may act as secondary mediators of HOCl/OCl−-induced injury. This study compared the effects of five N-chloroamino acids (AlaCl, LysCl, SerCl, AspCl and PheCl) on erythrocytes with the action of HOCl/OCl−. The N-chloroamino acids differed in stability and reactivity. They had a weaker haemolytic action than HOCl/OCl−; HOCl/OCl−, AlaCl and PheCl increased osmotic fragility of erythrocytes at a concentration of 1 mm. Oxidation of glutathione, formation of protein-glutathione mixed disulphides and efflux of GSSG from erythrocytes were observed for erythrocytes treated with all the employed chloroderivatives, while increased oxidation of 2′,7′-dichlorofluorescin was detected only after treatment of the cells with 1 mm HOCl/OCl−, AlaCl and PheCl. Generally, the reactivity of at least some N-chloroamino acids may be not much lower with respect to HOCl/OCl−.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
