Abstract
Calcineurin is a Ca2+/calmodulin dependent serine/threonine protein phosphatase, and has multiple functions in animal cells. In this work, mouse calcineurin was introduced into wild-type rice and the expression of calcineurin inhibited the induction and growth of rice calli. Inhibitor analysis showed that untransformed and CNAtr transgenic callus cultures had different sensitivity to cyclosporin A (CsA), a specific inhibitor of protein phosphatase calcineurin. When callus cultures were subject to 1 μM of CsA, the growth of calli induced from untransformed wild-type rice was inhibited. Interestingly, the growth inhibition of CNAtr transgenic calli was not detected in presence of 1 μM of CsA. Our findings showed that the heterologous calcineurin might be involved in the regulation of cell growth in plant cells.
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