Effect of MMS2 in the process of angiotensin II induced neural stem cells differentiated to dopaminergic neurons

Abstract

Objective To explore the possible effects of methyl methanesulfonate sensitive 2 （MMS2） in the process of angiotensin II inducing differentiation of neural stem cells （NSCs） into dopaminegic phenotype neurons. Methods NSCs were isolated from the brain of newborn rats and were cultured in the serum-free medium. Identification of neural precursor cells was done by Nestin immunocyt ochemical staining. Then the second generation of NSCs was divided into the following six groups： A, control; B, AII; C, AT1 antagonist ZD7155; D, ZD7155＋AII; E, AT2 antagonist PD123319; F, PD123319＋AII. The detection of expression of MMS2 and TH mRNA level was done by real-time PCR. The silence of the expression of MMS2 in NSCs was brought about via the transfection of MMS2-siRNA, and then the NSCs were induced to differentiate into dopaminegic neurons. The expression of TH mRNA level in the cells of the groups after transfeetion was detected by real-time PCR. Results Nestin-positive cells were observed in suspended growth in the medium. Real-Time PCR revealed that the MMS2 and TH mRNA expression of group B and D were significantly higher than that of the control group（P〈0.05 ）, There was no significant difference in MMS2 and TH mRNA expression between group C, E, F and the control, respectively. Conclusion AII increased the expression of MMS2 mRNA in NSCs and induced the differentiation of NSCs into DA neurons via AT2 recepter. MMS2 may play important roles in the process of angiotensin II inducing NSCs to differentiate into dopaminergic neurons. Key words: Neural stem cells; Differentiation; Dopaminergic neuron; Angiotensin II; Methyl methanesulfonate sensitive 2