Effect of miR-206 on the growth of bladder cancer cells and its mechanism

Abstract

Objective To investigate the effect of miR-206 on the growth of bladder cancer cell lines T24 and 5637 and its molecular mechanism. Methods According to the different treatment, bladder cancer cells were divided into control group (transfected with miR-NC) and experimental group (transfected with miR-206). EdU proliferation assay and colony-forming assay were performed to detect the proliferation ability of bladder cancer cells after transfection. Flow cytometry was used to detect the cell cycle distribution of bladder cancer cells after transfection. The expression of CDK4 and GAK was detected by real-time quantitative PCR (qRT-PCR) and Western Blot. Results EdU proliferation assay showed that the proliferation ability of bladder cancer cells transfected with miR-206 was significantly decreased. The colony formation assay showed that the number of colonies formed by the miR-206 transfected bladder cancer cells was lower than that of the miR-NC. The results of flow cytometry showed that the percentage of cells in S and G2 / M phase decreased significantly after miR-206 transfection, but the percentage of cells in G0 / G1 phase was significantly increased. The results of qRT-PCR and Western Blot showed that expressions of CDK4 and GAK in T24 and 5637 significantly decreased after transfection of miR-206 compared to miR-NC (P<0.01). Conclusions miR-206 significantly inhibits the growth of bladder cancer cells. The mechanism is that miR-206 inhibits the cell cycle progression of bladder cancer cells by interfering the expressions of CDK4 and GAK. Key words: Urinary Bladder Neoplasms; Cell Proliferation; Cell Cycle