Abstract
A flow cytometric method was used to investigate the effect of miconazole (MCZ) on yeast-form cells ofCandida albicans. Relative changes in electric potential of mitochondrial and cytoplasmic membranes were assessed by 3,3′-dihexyloxacarbocyanine iodide (diO-C6-(3)) and bis-(1,3-dibutyl-barbituric acid) trimethine oxonol (diBA-C4-(3)) stainings, respectively. WhenC. albicans was exposed to MCZ at 10 μg/ml (a fungistatic concentration) for 2 h, no change appeared in cytoplasmic membrane potential, which was revealed by constant fluorescence intensity of diBA-C4-(3)-stained cells. On the other hand, the cells lost the ability to accumulate diO-C6-(3) in mitochondria by MCZ treatment. Time- and dose-responses in fluorescence intensity reflected that MCZ affected the mitochondrial activity ofC. albicans.
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