Abstract
The metabolism of Cephalosporium acremonium grown in a complex medium supplemented with DL-methionine or inorganic sulfate was studied. More growth occurred in a sulfate medium than in a methionine medium. Methionine-grown cells had an increased rate of respiration, a higher rate of catabolism with acetate and glucose as substrate, and higher specific activities of certain respiratory enzymes than sulfate-grown cells. Labeled acetate and glucose were assimilated at a faster rate by methionine-grown cells than sulfate-grown cells. Taurine, cystathionine, and small quantities of four acidic compounds were present in the amino acid pool of methionine-grown cells, but they were not detected in the pool of sulfate-grown cells. The differences in metabolic activity of sulfate and methionine-grown cells are discussed in regard to cephalosporin C synthesis.
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