Abstract
Background: Malate involves in the citrate/malate and transhydrogenase cycles to provide precursors for docosahexaenoic acid (DHA) synthesis. The optimal strategy was investigated for increasing DHA production in Schizochytrium species during fermentation. Results: DHA production increased by 47% and reached 5.51 g/L when 4 g malate/L was added during the rapid lipid accumulation stage in shake-flasks culture. Inducing effects of malate was further investigated through the analysis of three kinetic parameters, including specific cell growth rate (μ), specific glucose consumption rate (q Glu ) and DHA formation rate (q DHA ). DHA concentration was enhanced through a novel fed-batch strategy to a maximum value of 30.7 g/L, giving a yield of 0.103 g DHA/g glucose and a productivity of 284 mg L - 1 h - 1 . Conclusion: A novel malate feeding strategy was developed that enhanced DHA yield and productivity of Schizochytrium species which may offer a desirable method for industrial applications. Normal 0 21 false false false EN-US ZH-CN X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:Tabla normal; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.5pt; mso-bidi-font-size:11.0pt; font-family:Calibri,sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-font-kerning:1.0pt; mso-ansi-language:EN-US; mso-fareast-language:ZH-CN;}
Highlights
Long-chain ω-3 polyunsaturated fatty acids (PUFAs), such as docosahexaenoic acid (DHA; C22:6), have been reported as effective in human nutrition and the prevention of conditions such as heart disease, high blood pressure and mood disorders [1,2,3]
Production of biomass, total lipid, and DHA were studied in shake-flask cultures of Schizochytrium sp
The malate addition period was based on earlier studies on Schizochytrium sp. [8]
Summary
Long-chain ω-3 polyunsaturated fatty acids (PUFAs), such as docosahexaenoic acid (DHA; C22:6), have been reported as effective in human nutrition and the prevention of conditions such as heart disease, high blood pressure and mood disorders [1,2,3]. Algae and fungi are abundant in DHA content have been explored as potential source of DHA [5]. Fermentation to decrease the costs of DHA production. The optimal strategy was investigated for increasing DHA production in Schizochytrium species during fermentation. Inducing effects of malate was further investigated through the analysis of three kinetic parameters, including specific cell growth rate (μ), specific glucose consumption rate (qGlu) and DHA formation rate (qDHA). Conclusion: A novel malate feeding strategy was developed that enhanced DHA yield and productivity of Schizochytrium species which may offer a desirable method for industrial applications
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