Abstract
Luteotrophic hormone acts on testicular interstitial cells, promoting the activation of several cellular events that culminate in steroids synthesis. Since the interstitial tissue include several cell types, purified Leydig cells were used in this work. Isolated interstitial cells from immature rats were purified through a 0–40% metrizamide gradient. Either LH, HCG or Bt 2-cAMP significantly stimulated the incorporation of [ 3H]uridine into RNA, when compared to control. The effect of HCG on RNA synthesis was developed within 30 min after the addition of the hormone and was dose-dependent. The maximum effect was attained with 10 mIU/ml of HCG. These results indicate that HCG/LH or Bt 2-cAMP but not FSH, promote an acute stimulation of RNA synthesis by Leydig cells from immature rats.
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