Effect Of Lutein from Chlorella pyrenoidosa INK On The Activities And Phagocytic Capacity On Peritoneal Macrophage Cells Of Mice Infected With Staphylococcus aureus

Abstract

Chlorella pyrenoidosa is a microalgae that produce lutein compounds that can stimulate an immune response. This study examined the crude lutein extracted from biomass C. pyrenoidosa INK as an immunomodulator through observation activity and phagocytic capacity of peritoneal macrophages of mice. This study uses twenty-eight mice were divided into 7 groups each comprising 4 replicates ie Group (I) normal controls, mice were untreated(II) a negative control, mice were induced by Staphylococcus aureus; (III) positive control, mice were induced by S. aureus and treatment of meniran extract (Phyllanthus niruri, stimuno®). The treatment group (IV-VII), mice were induced by S. aureus and treatment of crude lutein, respectively: 0.058 mg, 0.117 mg, 0.234 mg and 0.468 mg per 20 g of body weight. Lutein crude was given for 2 weeks and the next day the mice were injected bacterium S aureus. The results showed that crude lutein from C. pyrenoidosa INK act as immunomodulators that can stimulate an immune response as the dose increases. Treatment of crude lutein dose of 0.234 mg per 20 g BW of mice showed a proportional response to the positive control treatment (stimuno®). Lutein of C.pyrenoidosa had a positive effect on the the activity and capacity of peritoneal macrophage in mice after stimulation with S. aureus bacteria.