Abstract
Objective To investigate the production of TFG-β1, VEGF and IL-6 by human hepatocellular carcinoma cells HepG-2 activated by LPS. Methods The levels of TGF-β1, VEGF, IL-6 mRNA and protein before and after the treatment of LPS were determined by RT-PCR and Western-blot. After inhibition of the TLR4, their levels were determined again. Results The levels of TGF-β1, VEGF, IL-6 mRNA and protein were all upregulated after the treatment of human hepatocellular carcinoma cells HepG-2 by LPS and then downregulated after the inhibition of TLR4. Conclusion As the exogenous ligand of Toll-like receptor 4, LPS can promote the production of pro-inflammatory cytokines TGF-β1, VEGF and IL-6 in human hepatocellular carcinoma cells. Key words: Carcinoma,hepatocellular; HepG-2; LPS; TLR4; RT-PCR; Western blot
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
