Abstract
To evaluate the effect of low-level laser therapy (LLLT) ë904 nm on healing of surgical wounds in rats. Forty male Wistar rats were used, divided into four groups, underwent incision along the lines Alba covering skin, subcutaneous and muscle abdominal, sutured continuously for nylon 5-0. Eight and fifteen days after the surgery process, the repairing area was removed and histological sections were stained with hematoxylin-eosin to assess cellularity inflammatory, Masson's Trichrome and Picrossirus to quantify the collagen fibers and immunohistochemical technique for counting newly formed vessels. The data were compared statistically using analysis of variance ANOVA, with a "post-hoc Tukey test, p <0.05. Low-level laser therapy reduced the intensity of the inflammatory reaction and influenced the dynamic of the immunoinflammatory response by inducing switching of the leukocyte infiltration pattern (neutrophilic to lymphoplasmacytic infiltration). Also stimulate the deposition and enhance the organization of collagen fibers, featuring a delicate collagen type III. Furthermore, it appeared to a significant increase in the average number of newly formed vessels (p = 0.00 and p = 0.02, respectively). Low-level laser therapy resulted in modulate of the inflammatory response, enhanced deposition of collagen fibers and increase in the average number of newly formed vessels.
Highlights
The discontinuity of tissue integrity can be induced either by trauma or by surgical procedures
Due to the extensive controversy observed in the literature concerning the role played by phototherapy on stromal and inflammatory cells involved in the cicatricial repair, the goal of this study is to analyze the effect of a specific protocol of low-energy Laser irradiation on the intensity of the inflammatory response, deposition of collagen fibers and number of newly formed vessels during two different phases of wound healing in rodents
The sample consisted of 40 adult male albino Wistar rats (Rattus norvegicus albinus), with body weight ranging from 200 ± 20 grams, from the animal colony of the Federal University of Sergipe (UFS), Sao Cristovao, State of Sergipe, Brazil
Summary
The discontinuity of tissue integrity can be induced either by trauma or by surgical procedures In this regard, numerous chemicals are released into the injured area causing tissue changes consistent with an acute inflammatory process. Numerous chemicals are released into the injured area causing tissue changes consistent with an acute inflammatory process This phenomenon is characterized by stimulation, migration and proliferation of stromal cells, synthesis of extracellular matrix, and culminating in tissue remodeling[1,2,3]. The LLLT has been clinically used for pain control and wound healing The effectiveness of this therapy is due to responses induced by the cellular level photobiomodulation promoting acceleration of cellular metabolism, reducing inflammation, increasing local microcirculation and the lymphatic system, leading to the decrease of the interstitial edema and increase of the collagen synthesis[4,5,6,7]
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