Abstract
ObjectiveThis study aims to investigate the effect of long non-coding RNA (lncRNA) Gas5 on proliferation, migration, invasion and apoptosis of colorectal cancer (CRC) HT-29 cell line.MethodsCRC and normal tissues were collected and prepared from a total of 126 CRC patients, and normal intestinal epithelial cell line FHC and CRC cell lines (HCT-8, HT-29, HCT-116 and SW-480) were prepared. Gas5 expression was detected by quantitative reverse transcriptase-polymerase chain reaction. HT-29 cell line exhibiting the lowest Gas5 expression was selected for further experimentation and divided into blank, negative control and pcNDA-Gas5 groups. The cell counting kit-8 assay was used to test cell proliferation. Flow cytometry was applied to examine cell apoptosis. Transwell assay was performed to detect the migration and invasion of HT-29 cells. The mRNA and protein expression of factors in the classical proliferation (Akt/Erk) and apoptosis (caspase-9/caspase-3) pathways were detected.ResultsGas5 expression was lower in CRC tissues compared to the adjacent normal tissues, and is also lower in CRC cell lines than FHC cell line. Gas5 expression was associated with tumor size and TNM staging. Gas5 expression, distant metastasis, tumor differentiation and TNM staging were independent CRC prognostic factors. The results showed that elevated Gas5 expression inhibited proliferation, migration and invasion, but promoted apoptosis of CRC cells. Meanwhile, elevated Gas5 expression inhibited mRNA expression of Akt and Erk and protein expression of p-Akt and p-Erk, which promoted Casp9 mRNA and pho-Casp9 protein expression but inhibited Casp3 mRNA and pho-Casp3 protein expression.ConclusionThe findings indicated that overexpression of lncRNA Gas5 can inhibit the proliferation, migration and invasion but promote apoptosis of CRC cells.
Highlights
Colorectal cancer (CRC) is the most common cause of cancer-related death across the world [1]
growth arrest-specific transcript 5 (Gas5) mRNA expression in CRC cell lines (HCT-8, HT-29, HCT-116, SW-480) was 1.11 ± 0.16, 0.83 ± 0.14, 1.31 ± 0.14 and 1.38 ± 0.15, lower compared to the normal colonic epithelial cell line FHC (2.35 ± 0.40) (P < 0.05, Fig. 1b)
Protein expression of Gas5 in adjacent normal tissues was significantly higher compared to the CRC tissues (P < 0.05, Fig. 1c, e), and Gas5 protein expression in CRC cell lines was lower compared to the normal colonic epithelial cell line (P < 0.05, Fig. 1d, f )
Summary
Colorectal cancer (CRC) is the most common cause of cancer-related death across the world [1]. More than 50% CRC cases occur in developed countries [2]. Li et al Cancer Cell Int (2018) 18:4 impairment of different signaling pathways [8]. Substantial improvements have been made in the diagnosis and treatment of CRC [9]. CRC incidence and mortality have decreased in recent years, the 5-year survival rate of CRC patients remains low [10] An early and accurate diagnosis as well as a precise evaluation of the survival post-operation would greatly improve the treatment for CRC [2]. A candidate molecular biomarker is required for patients with CRC
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