Abstract
Lipoxygenases (LOXs, EC 1.13.11.12), which effected the quality of soybean, rice and maize in storage processing, is the key enzyme in seed lipid metabolism ways, but is rarely reported in peanut. In order to determine the activity of LOXs in peanut, a quantitative method for the determination of LOX3 activity in peanut was developed, which was optimized on the basis of the method of Lox3 Beta-carotene coupling colorimetry. The optimum sample size was 8 mg, and the optimum time was 10–70s after reaction. The distribution of LOX3 activity of 214 peanut germplasm resources was studied by this method, which showed that the difference of enzyme activity between the materials was very significant P < 0.01, and 24 peanut germplasm with obvious difference of LOX3 activity were propagated. The results of 18 months natural storage aging test showed that the LOX3 activity of peanut was positively correlated with its seed activity (Correlation Coefficient was 0.6984, P < 0.01), and the storage life of high LOX3 activity peanut was longer. The results provided a basis for further study on the correlation between Lipoxygenase and storage characteristics of peanut seeds.
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