Abstract
The effect of mechanical treatment on the denaturation of cellulolytic enzymes was studied. Mechanical treatment of the enzyme complex together with the lignocellulose substrate makes it possible to decrease the rate of denaturation and retain most of enzymes in the active form. A possible reason for increasing the stability to mechanical treatment is amorphization of the cellulose component of the lignocellulose substrate and, as a consequence, decrease in its hardness. For retaining the catalytic activity, it is necessary to reduce as much as possible the residence time of the enzyme in the mechanical treatment zone.
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