Abstract
Culex quinquefasciatus mosquitoes are a vector of transmission of several types of diseases, such as filariasis, Japanese encephalitis, and dirofilariasis. Larval control is the key strategy of disease control programs caused by vectors around the world because it can prevent larvae to enter the adult stage. Control of mosquito larvae that are often used is chemically controlled. Citrus hystrix is a natural plant and contains chemical compounds that have biological activity, such as flavonoids, carotenoids, and limonoids. The present study aimed to determine the effect of citrus leaf extract (Citrus hystrix) on the mortality of the larva Culex quinquefasciatus. The method used in the current study was a laboratory experimental study method with the experimental design using a completely randomized design. The research plot carried out was the rearing larvae of Culex quinquefasciatus, the manufacture of citrus leaf extract, the manufacture of larvicidal material, and the observation phase after treatment. Extract N-hexane omplet name hystrix leaf has high toxicity because it contains essential oils, flavonoids, alkaloids, terpenoids, saponins, and Limonoids. The Optimal concentration of N-hexane extracts of citrus leaves that lead to larval mortality of Culex quinquefasciatus in vitro amounted to 93.33% on 4 hours of observation with a concentration of 4000 ppm. Extract N-hexane lime leaf has a larvicidal effect on the larva Culex quinquefasciatus in vitro.
Highlights
Indonesia is a tropical country that has optimal humidity and temperature so as to support insect survival
Culex (Cx.) quinquefasciatus mosquitoes are the vector of transmission of several types of diseases such as filariasis, Japanese encephalitis, and dirofilariasis (Hadi and Koesharto, 2006)
Japanese Encephalitis (JE) is a viral disease that causes encephalitis and is a problem of public health in Asia including in Indonesia
Summary
Indonesia is a tropical country that has optimal humidity and temperature so as to support insect survival. Culex (Cx.) quinquefasciatus mosquitoes are the vector of transmission of several types of diseases such as filariasis, Japanese encephalitis, and dirofilariasis (Hadi and Koesharto, 2006). Control of mosquito larva that has been often used is chemical control, namely by using synthetic chemicals Control in this way when done repeatedly is less effective because it can cause resistance to larvae, death for animal predatory predators and environmental pollution (Yasmin and Fitri, 2013). Effect of Larvicidal Extract N-Hexane Lime Leaves (Citrus hystrix) on Larva Instar III Mosquito (Culex quinquefasciatus). Limonoid compounds are the teranoriterpen found in the leaves of Citrus lime which is potentially antifeedant to insects, growth regulator, and toxic substances in rice lice, larvacide, antimicrobial, insect repellent, and reproductive inhibitors (Li, 2001). This research is done to find out the benefits of citrus leaves that are thought to have effects on larvae of Cx. quinquefasciatus
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