Effect of lactobacillus salivarius on asthma Balb/c mouse in IL-4, IFN-γ and IgE of serum and lung tissue homogenates

Abstract

Objective To invstigate the effect of Lactobacillus salivarius on asthma Balb/ c mouse in IgE, IL-4 and IFN-γ of serum and lung tissue homogenates. Methods Thirty female Balb/c mice weighing 16-18 g (4 weeks old), SPF grade, were randomly divided into three groups: normal control group (N), asthma group(A), asthma combined Lactobacillus salivarius group (AH). Acute asthma model was established by ovalbumin challenge method, mice were observed in behavioral changes, non-invasive method for the determination of airway responsiveness in mice, the changes in airway inflammation and the number changes of tracheal cilia were observed by pathological HE staining and the electron microscope.Respectively, the levels of IgE and IL-4/IFN-γ in the serum and lung tissue homogenate were measured by ELISA. Results five~ten min after 4%OVA saline solution atomization excitation mice in A group appeared obvious restlessness, head and facial itching, scratching, shortness of breath, perioral cyanosis with abdominal breathing, sneezing, stooping, forelimbs shrinkage, urine and feces incontinence and other asthma acute phase performances, The above reaction alleviated in AH group compared to A group; Pathology of HE staining in asthma group showed the thickening bronchial wall, narrow lumen, peribronchial and perivascular infiltration with a large number of eosinophil-based inflammatory cells, lumen with many inflammatory secretions, Pathological changes in lung tissue of mice in AH group significantly alleviated compared with asthma group; Under the electron microscope, the number of cilia in A group significantly decreased compared with N group, while AH group significantly increased compared with A group; Expression level of IgE, IL-4 in the serum and lung homogenate of A group was significantly higher than that of N group(P<0.05), the level of IFN-γ expression was significantly lower than N group(P<0.05), however the expression level of IgE, IL-4 in AH group significantly decreased compared with A group(P<0.05), and IFN-γ expression level elevated compared with A group(P<0.05). Conclusion Lavage with Lactobacillus salivarius before sensitization could alleviate clinical symptoms of asthmatic mice, partially correct Th1/Th2 imbalance as well as reduce airway inflammation. Key words: Lactobacillus salivarius; Asthma; IgE; IL-4; IFN-γ; Mouse