Effect of Iron on Some Parameters Recombinant Pseudomonas aeruginosa Carrying Vitreoscilla Hemoglobin Gene

Abstract

In this study, the effects of iron presence on some bacterial parameters of Pseudomonas aeruginosa and its recombinant bacteria carrying Vitreoscilla hemoglobin on its chromosome were investigated for the first time. These parameters are; optical density, pH, glucose, trehalose production and biomass. Parameters; It was studied at 37 °C and 250 rpm ventilated conditions depending on time. Bacteria have developed mechanisms by which they can resist heavy metal stress with many other mechanisms, including making metals less toxic and excreting them out of the cell. The Efflux system is the most widely used mechanism. The bacterium that makes the best use of these mechanisms is P. aeruginosa, which has an environmental and versatile feature. In the presence of LB alone, an increase was observed in the first 48 hours and a decrease of 43% in the other time periods, especially in the 96th hour compared to the control. The highest increase was detected in the 48th time periods, up to 259% in the 3,32. When iron was added to the medium, significant increases were observed in all time periods compared to the controls and these increases reached 575% at 72 hours. In the same time periods, the maximum value of OD600 4.55 was reached.