Abstract
Objective: To observe the changes of endothelial mitochondrial membrane potential（MMP）by building Hcy-induced endothelial impairment model in cultured human umbilical vein endothelial cells and to analyze the mechanism of Hcy-induced apoptosis. Methods: (1) Cultured HUVECs of term infant in vitro provide enough experimental materials. (2)The HUVECs are randomly divided into five groups: the control group does not add Hcy, the other groups add 0.01、0.1、1.0、3.0mmol/LHCY respectively, and put them together for 24 hours to detect the changes of MMP of the HUVECs at 3、6、12、24 hour. Results: There is no difference in the level of MMP in the HUVECs stimulated by HCY for 24 hours between the HCY0.01mmol/L group and the control group. Comparing with the control group,MMP reduces at 24 hour in the HCY0.1 mmol/L group ,at 6、12、24 hour in the HCY1.0 mmol/L group and at 3、6 hour( P <0.05）and obviously at 12、24 hour( P <0.01) in the HCY3.0 mmol/L group.In the same Hcy-induced group, MMP obviously reduces at 24 hour, ＞12h、6h、3h.At the same time, MMP obviously reduces at 3.0mmol/L Hcy-induced group, ＞1.0、0.1、0.01 mmol/L group and the control group. Conclusion: (1)HCY can injury endothelial function and induce apoptosis and it relates with concentration and time of HCY.(2) Mechanism of Hcy- induced endothelial apoptosis may relates the reduction of MMP.
Highlights
2)The HUVECs are randomly divided into five groups
together for 24 hours to detect the changes of MMP of the HUVECs
There is no difference in the level of MMP
Summary
【摘要】目的 探讨同型半胱氨酸诱导人脐静脉内皮细胞线粒体膜电位(MMP)的变化,了解血管内皮细胞发生细胞凋亡的机 制。方法 无菌采集正常足月剖腹产胎儿脐带进行人脐静脉内皮细胞培养,取对数生长的人脐静脉内皮细胞制成细胞悬液, 加含 1%胎牛血清(FCS)的培养基(DMEM)处理细胞 24h,使细胞稳定、同步化。随机分成五组:(1).空白组:仅加含 1% FCS 的 DMEM5ml。(2) HCY0.01mmol/L 组:1%FCS 的 DMEM+HCY 终浓度为 0.01 mmol/L 5ml。(3.)HCY0.1 mmol/L 组:1%FCS 的 DMEM+HCY 终浓度为 0.1mmol/L 5ml。(4).HCY1.0 mmol/L 组:1%FCS 的 DMEM+HCY 终浓度为 1.0 mmol/L 5ml。(5)HCY3.0mmol/L 组:1%FCS 的 DMEM+HCY 终浓度为 3.0 mmol/L 5ml。继续培养 24h,每组 3 个样本。结果 不 9 同浓度及时间 HCY 对 HUVECs 线粒体膜电位的影响:MMP 在 HCY0.01mmol/L 组和空白对照组间无差异性变化;在 HCY0.1 Journal.newcenturyscience.com/index.php/gjccd mmol/L 组于 24h 时出现下降(P
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