Abstract
Summary Uptake studies were carried out with intact peribacteroid units isolated from nodules of soybean plants (Glycine max L. cv. Hodgson) inoculated with Bradyrhizobium japonicum strain 273 and treated for 48h with high concentrations of nitrate. Malate penetrated the peribacteroid membrane most rapidly when the plants were affected by 0.390 mM nitrate. The 2 mM nitrate treatment had a slightly negative effect on the malate uptake, resulting in a 12 % decrease in the dicarboxylate transport across the peribacteroid membrane, as compared with the controls. Malate transport across the peribacteroid membrane decreased by 55% after 7.5 mM nitrate treatment and by 85% after 15 mM nitrate treatment. At higher nitrate levels poly-β-hydroxybutyrate synthesis was increased. These data support the suggestion that in these conditions malate is funneled to poly-s-hydroxybutyrate synthesis, which together with the reduced malate uptake by peribacteroid units could explain the inhibited nitrogen fixation.
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