Abstract
Pineapple crowns (Ananas comosus Smooth Cayenne') known to be infected with a closteroviruslike particle associated with mealybug wilt of pineapple were heat-treated in water at 40, 50, and 60 C for 30, 60, and 120 min at each temperature. Plant survival was 80-100% at 40 and 50 C, and enzyme-linked immunosorbent assay (ELISA) demonstrated that 60-100% of these surviving plants were rendered free of closteroviruslike particles. Heat treatment at 60 C increased plant mortality and did not improve the percentage of plants rendered free of virus (...)
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