Abstract
AbstractThis study was performed to elucidate the potential influence of He Qi San (HQS) on glucose and lipid metabolism in type 2 diabetes mellitus (T2DM) patients with phlegm-blood stasis syndrome (PBSS), and to determine DNA methylation changes. Sixty T2DM patients with PBSS were randomly divided into control and HQS groups. The control group received conventional treatments, and the HQS group received conventional treatments plus HQS. Glucose metabolism (FPG, 2hPG, FINS, and HbA1c) and lipid metabolism indexes (TG, TC and LDL-C) were determined. Genes with differential DNA methylation were subjected to GO and KEGG analyses. Glucose and lipid metabolism indexes in both groups were reduced, but were much more pronounced in the HQS group. Differential promoter CpG methylation regions were identified in 682 genes, including 426 genes with high-CpG promoters, 150 genes with intermediate CpG promoters, and 106 genes with low CpG promoters. Genes with differential DNA methylation were mainly enriched in the AMPK and insulin signaling pathways, terpenoid backbone biosynthesis, and renin secretion. We concluded that HQS remarkably improved indexes of glucose and lipid metabolism in T2DM patients with PBSS through regulating the DNA methylation of genes in the AMPK and insulin signaling pathways and terpenoid backbone biosynthesis.
Highlights
Diabetes is a metabolic disease characterized by persistent elevation of the blood glucose level
Previous studies in our laboratory have indicated that He Qi San (HQS) is capable of reducing body weight and decreasing the appetite of type 2 diabetes mellitus (T2DM) patients accompanied by obesity [5, 6]
T2DM is characterized by insulin resistance with a relative or absolute decline of pancreatic islet function
Summary
Diabetes is a metabolic disease characterized by persistent elevation of the blood glucose level. Traditional Chinese medicine (TCM) presents great efficacy and distinctive treatment characteristics in the long-term prevention and treatment of diabetes [3] He Qi San (HQS) is an efficacious prescription that is used by the Endocrinology Department, Shenzhen TCM Hospital on the basis of years of clinical experience [4]. Previous studies in our laboratory have indicated that HQS is capable of reducing body weight and decreasing the appetite of type 2 diabetes mellitus (T2DM) patients accompanied by obesity [5, 6]. It alleviates insulin and leptin resistance in patients with metabolic syndrome, corrects chronic inflammatory states, and decreases peripheral insulin sensitivity [6, 7]. HQS has a strong effect on pre-diabetes, but its specific molecular mechanism remains unclear
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