Abstract
The present study was carried out to explore the effects of graphene oxide (GO) on callus induction, differentiation, metabolite biosynthesis and antioxidant capacity for tobacco leaves. Results showed that lower concentrations of GO (lower than 600 μg/mL) stimulate the differentiation of tobacco callus into buds and leaves. The content of chlorophyll, soluble sugar, soluble protein, and the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) all increased at lower concentrations of GO. In addition, the length of roots and stems of the regenerated tobacco callus also increased. However, GO induced an increase in malondealdehyde (MDA) content. The experimental results could provide valuable reference for other researches about interactions between carbon-based nanomaterials and plant in callus formation differentiation, metabolite biosynthesis and nanometer pollution.
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