Abstract
The effect of different doses of gamma rays on the fresh weight and some cell constituents of Black seed (Nigella sativa L.) stem callus was investigated. The results revealed an increase in the fresh weight with an increase in the cellular contents of protein and nucleic acid (DNA, RNA) extracted from radiated callus at doses 5,10, 30 and 50 rad, on the other hand, the high doses (70, 90 rad) led to death more than of 50% of initiating cells of callus. Shoots formation occurred on radiated callus, at dose 50 rad. In solid MS media, which are provided with 2,4–D(10-6 M). Verdure branches that occurred on radiated callus have been rooted by using MS free growth regulators. The plant developed on this medium can be readly transfered to the soil after harding.
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