Abstract
Objective To explore the effect of gambogic acid (GA) on the growth activity and invasion in renal carcinoma 786-O cells.Methods After treatment with different concentrations of GA for 24 h,the cell viability and invasion ability were measured by methyl thiazol tetrazolium (MTT) assay,flow cytometry and invasion assay,respectively.The protein expression of bax and matrix metalloproteinase ( MMP)-9 were detected by using Western blotting.Results In comparison with controls,the 1.5,3.0 and 6.0 μmol/L GA group could inhibit the cell proliferation,and induce the cell apoptosis.In the 1.5,3.0 and 6.0 μmol/L GA groups,the 1.5,3.0 and 6.0 μmol/L GA group The inhibition rate of cell growth was ( 18.45 ± 3.41 ) ％,( 27.88 ± 6.24) ％,( 50.48 ± 12.13 ) ％ ( P ＜ 0.05 ),and the apoptosis rate was (21.15 ± 1.68 ) ％,( 33.57 ± 6.35 ) ％,(46.33 ± 9.74 ) ％ ( P ＜ 0.05 ) respectively.In addition,the number of invasion cell was decreased significantly after treatment with 0.375 and 0.750 μmol/L GA.Moreover,the expression of bax protein was up-regulated and the MMP-9 was down-regulated in GAtreated groups.Conclusion GA could not only suppress renal cell carcinoma 786-O cell growth but also inhibit the cell invasion,which may be associated with the expression of bax and MMP-9. Key words: Gambogic acid; Renal carcinoma; Cell invasion
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