Abstract
Objective To establish a rat model of spinal cord injury (SCI) and evaluate the effect of in vivo treatment of SCI with FGL-NS peptide that could be self-assembled in vivo into functionalized nanofiber scaffold.Methods The rats were anesthetized and treated with laminectomy at the T10 vertebral segment.Then,the SCI models were established by compressing the spinal cord with a 24 g modified aneurysm clip.One day after SCI,the animals were respectively injected with 2.5 μl 580 μmol/L solution of glucose,1％ RADA-16 and 1％ FGL-NS peptide solution into the injured zone.After treatment for 3 days,1 week,3 weeks,5 weeks,7 weeks and 9 weeks,the functional recovery of SCI rats was evaluated by the Basso,Beattie and Bresnahan Locomotor Rating Scale.After treatment for 9 weeks,apoptosis rate and the number of neurons with neurite and astrocyte were detected by Caspase-3,neurofilament-200 (NF-200) and glial fibrillary acidic protein (GFAP) immunohistochemistry.Results The model of SCI in rats was established successfully.The Basso-Beattie-Bresnahan (BBB) scores of SCI rats treated with FGL-NS were higher than those in RADA-16 group after treatment for 5 weeks.The number of Caspase-3 positive cells in injuried zone treated with FGL-NS was (22.45 ± 2.74) per field,which was significantly less than in RADA-16 group [(30.86 ±3.75) per field].Meanwhile,the number of neurons with neurite in FGL-NS group was (35.32 ±3.12) per field,significantly more than that in RADA-16 group [(18.56 ±2.64) per field] and blank group [(14.83 ± 1.43) per field].Furthormore,FGL-NS inhibited the formation of astrogila at the site of injury.The integrated option density (IOD) in injuried zone treated with FGL-NS was 0.50 ± 0.02,significantly less than in control group (1.30 ± 0.09) and blank group (1.60 ± 0.11).Conclusion Functionalized peptide self-assembling nanofiber scaffolds containing FGL motif could be a useful biomaterial in nerve tissue engineering for improving the functional recovery of SCI rats,inhibiting the formation of astrogila,reducing the cell death and increasing the number of neurons in the injured zone. Key words: Spinal cord injury ; Self-assembly; Regeneration ; Tissue engineering; Scaffold
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