Abstract

Measurements of the rate of nitrogen excretion were carried out with fed and starved Japanese flounder of 163 to 575g in body weight at 20°C. Ammonia-N and urea-N excretion of the starved fish were 0.20±0.05mg-N/100g/h and 0.04±0.02mg-N/100g/h, respectively. Immediately after feeding, the rate of ammonia-N excretion per hour of the flounder became 3 to 4 times that of the starved fish. These elevated rates continued for 12, 30 and more than 36h, at 0.5, 1.0 and 1.5% rations, respectively. In contrast to ammonia-N excretion, the rate of urea-N excretion per hour of the fed fish showed a major peak during the period of 6-12 or 12-24h after feeding. In Japanese flounder fed on the pelleted diet containing 7.5% of nitrogen at average ration of 0.5%, it is estimated that 32% of the consumed nitrogen was excreted as ammonia-N, about 5% as urea-N and 8% as faeces-N within 24h after feeding.

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