Effect of Ethanol Extract from Kepel Leaves (Stelechocarpus burahol (Bl.) Hook F. & Th.) on Confluence and Viability of Primary Mouse (Mus musculus) Neuronal Cells

The aim of the study was to investigate antihyperuricemic effect of snake fruit (Salacca edulis Reinw.) var. Bongkok Wistar male rates. Antihyperuricemic investigation on Wistar male rats showed that administration of ethanol extract at doses of 200 mg/kg bw decreased serum uric acid level significantly compared to control group at hour 6 and 7 (P < 0.05) after inducing with potassium oxonate intraperitoneally simultaneously with uric acid orally. Whereas, administration of ethanol extract at doses of 100 mg/kg bw did not decrease serum uric acid level significantly different compared to control group at hour 6 and 7 (P < 0.05). Determination of uric acid level in urine, administration of ethanol extract at a dose of 200 mg/kg bw, or probenecid as a standard drug, at a dose of 45 mg/kg bw increased excretion of urine uric acid level significantly different compared to control group in day of 7 (P < 0.05) after inducing with potassium oxonate intraperitoneally simultaneously with uric acid orally. However, increase of uric acid excretion by ethanol extract was lower compared to that of probenecid at a dose of 45 mg/kg bw. Mechanism of action of the ethanol extract as an antihyperuricemia has been proposed by inhibition of xanthine oxidase and finally decreased the synthesis of uric acid and increased the excretion of urine uric acid level.

Effects of Perfluoroalkyl Compounds on mRNA Expression Levels of Thyroid Hormone-Responsive Genes in Primary Cultures of Avian Neuronal Cells

Introduction: This study aimed to investigate the preventive effect of the ethanolic extract of galangal (Alpinia galanga) on degeneration, necrosis, and inflammatory cells in the kidney proximal tubules of male mice (Mus musculus) exposed to lead acetate. Objective: This was a pure experimental laboratory study using a completely randomized approach/randomized posttest only control group design. Twenty-five male mice (Mus musculus) were divided into five treatment groups: negative control (K-) received 0.5% CMC Na and aquades, positive control (K+) received induction of 20 mg/kgBW lead acetate for 20 days, and P1, P2, and P3 groups received oral administration of galangal ethanol extract from day 1 to day 24 as preventive measures with successive doses of (P1) 200 mg/kgBW, (P2) 400 mg/kgBW, and (P3) 800 mg/kgBW, respectively. Lead acetate administration at a dose of 20 mg/kgBW started from day 4 to day 24. On the 25th day, all groups were sacrificed, and kidney tissues were harvested for histopathological examination using the scoring method. Histopathological data analysis was performed using the Kruskal-Wallis test followed by Mann-Whitney U test to compare differences between groups. Results: The Mann-Whitney U test showed a significant increase in degeneration, necrosis, and infiltration of inflammatory cells in the kidney proximal tubules of male mice (Mus musculus) exposed to lead acetate (p &lt; 0.05). Administration of galangal ethanol extract significantly reduced degeneration, necrosis, and infiltration of inflammatory cells (p &lt; 0.05). The highest dose of galangal ethanol extract (800 mg/kg BW) exhibited a significant decrease in degeneration, necrosis, and infiltration of inflammatory cells (p &lt; 0.05), comparable to the normal condition (K-). Conclusion: Administration of ethanol extract of galangal (Alpinia galanga) effectively prevented lead acetate-induced degeneration, necrosis, and infiltration of inflammatory cells in the kidney proximal tubules of male mice (Mus musculus).

Indonesia has a tropical climate is rich in flora diversity. Many medicinal plants, including the Kepel (Stelechocarpus burahol), are herbal medicines. Kepel Fruit (Stelechocarpus burahol) contains secondary metabolites of alkaloids, flavonoids, polyphenols, saponins, triterpenoids and quinones. Some of these compounds have anti-fertility properties. Antifertility compounds are compounds that can prevent fertility by interfering with several normal reproductive mechanisms, both in men and women. This study aims to analyze the effectiveness of Kepel fruit extract on the growth and development of secondary spermatocytes in mice (Mouse muscle). This research uses true experiments and Posttest-Only Control with a quantitative approach. The samples used for this research design, both experimental and controlled, were taken randomly. Testing the significance of the treatment effect uses parametric statistics, namely the One-Way ANOVA test at a significance level of 5% (α = 0.05). Then, if there are differences, continue with the LSD further test (Least Significant Difference) at a significance level of 5% (α = 0.05). This research design used a Randomized Block Design (RAK) with a total sample of 32 mice (Mouse muscle) with 8 repetitions and 3 treatment groups. The growth and development of secondary spermatocytes in mice are determined by counting the number of secondary spermatocytes in the seminiferous tubules of mouse testicular incisions. The results of the one-way Anova test show that the calculated F is 6.41, and the value is sig. (P.Value) of 0.002, these results suggest P.Value (0.002) &lt; the α value (0.05), which means that the number of secondary spermatocytes is significantly different in each group so that this study can conclude that the administration of Kepel fruit extract (Stelechocarpus burahol) can significantly reduce the number of growth and development of mouse secondary spermatocytes (Mouse muscle ).

Taurine is an inhibitory neurotransmitter and is one of the most abundant amino acids present in the mammalian nervous system. Taurine has been shown to provide protection against neurological diseases, such as Huntington's disease, Alzheimer's disease, and stroke. Ischemic stroke is one of the leading causes of death and disability in the world. It is generally believed that ischemia-induced brain injury is largely due to excessive release of glutamate resulting in excitotoxicity and cell death. Despite extensive research, there are still no effective interventions for stroke. Recently, we have shown that taurine can provide effective protection against endoplasmic reticulum (ER) stress induced by excitotoxicity or oxidative stress in PC12 cell line or primary neuronal cell cultures. In this study, we employed hypoxia/reoxygenation conditions for primary cortical neuronal cell cultures as an in vitro model of stroke as well as the in vivo model of rat focal middle cerebral artery occlusion (MCAO). Our data showed that when primary neuronal cultures were first subjected to hypoxic conditions (0.3%, 24 h) followed by reoxygenation (21%, 24-48 h), the cell viability was greatly reduced. In the animal model of stroke (MCAO), we found that 2 h ischemia followed by 4 days reperfusion resulted in an infarct of 47.42 ± 9.86% in sections 6 mm from the frontal pole. Using taurine greatly increased cell viability in primary neuronal cell culture and decreased the infarct area of sections at 6 mm to 26.76 ± 6.91% in the MCAO model. Furthermore, levels of the ER stress protein markers GRP78, caspase-12, CHOP, and p-IRE-1 which were markedly increased in both the in vitro and in vivo models significantly declined after taurine administration, suggesting that taurine may exert neuroprotection functions in both models. Moreover, taurine could downregulate the ratio of cleaved ATF6 and full-length ATF6 in both models. In the animal model of stroke, taurine induced an upregulation of the Bcl-2/Bax ratio and downregulation of caspase-3 protein activity indicating that it attenuates apoptosis in the core of the ischemic infarct. Our results show not only taurine elicits neuroprotection through the activation of the ATF6 and the IRE1 pathways, but also it can reduce apoptosis in these models.

Taurine has been shown to provide protection against neurological diseases, such as Huntington's and stroke. We have shown that taurine can provide effective protection against Endoplasmic Reticulum (ER) stress induced by excitotoxicity or oxidative stress in PC12 cell line or primary neuronal cell cultures. In this study, we employed hypoxia/reoxygenation conditions for primary cortical neuronal cell cultures as well as the model of rat focal Middle Cerebral Artery Occlusion (MCAO). Using taurine greatly increased cell viability in primary neuronal cell culture and decreased the infarct area of sections at 6mm from 47.42±9.86% to 26.76 ± 6.91% in the MCAO model. Furthermore levels of the ER stress protein markers GRP78, Caspase12, CHOP and p‐IRE‐1 which were markedly increased in both the in vitro and in vivo models, significantly declined after taurine administration, suggesting that taurine may exert neuroprotection functions in both models. Moreover, taurine could downregulate the ratio of cleaved ATF6 and full length ATF6 in both models. In the animal model of stroke, taurine induced an upregulation of the Bcl‐2/Bax ratio and downregulation of Caspase‐3 protein activity indicating that it attenuates apoptosis in the core of the ischemic infarct. Our results show not only taurine elicits neuroprotection through the activation of the ATF6 and the IRE1 pathways, but also it can reduce apoptosis in these modelsGrant Funding Source: 09KW‐11, Department of Health, State of Florida

Cannabis is considered (Cannabis sativa L.) a sacred herb in many countries and is vastly employed in traditional medicine to remedy numerous diseases, such as diabetes. This research investigates the chemical composition of the aqueous extracts from Cannabis sativa L. seeds. Furthermore, the impact of these extracts on pancreatic α-amylase and lipase, and intestinal α-glucosidase enzymes is evaluated, as well as their antihyperglycemic effect. Analysis of the chemical composition of the aqueous extract was conducted using high-performance liquid chromatography with a photodiode array detector (HPLC-DAD). In contrast, the ethanol, hexanic, dichloromethane, and aqueous extract compositions have been established. Additionally, the inhibitory effects of ethanolic, dichloromethane, and aqueous extracts on pancreatic α-amylase and lipase, and intestinal α-glucosidase activities were evaluated in vitro and in vivo. The results of HPLC analysis indicate that the most abundant phenolic compound in the aqueous cannabis seed extract is 3-hydroxycinnamic acid, followed by 4-hydroxybenzoic acid and rutin acid. Moreover, administration of ethanolic and aqueous extracts at a dose of 150 mg/Kg significantly suppressed postprandial hyperglycemia compared to the control group; the ethanolic, dichloromethane, and aqueous extracts significantly inhibit pancreatic α-amylase and lipase, and intestinal α-glucosidase in vitro. The pancreatic α-amylase test exhibited an inhibition with IC50 values of 16.36 ± 1.24 µg/mL, 19.33 ± 1.40 µg/mL, 23.53 ± 1.70 µg/mL, and 17.06 ± 9.91 µg/mL for EAq, EDm, EET, and EHx, respectively. EET has the highest inhibitory capacity for intestinal α-glucosidase activity, with an IC50 of 32.23 ± 3.26 µg/mL. The extracts inhibit porcine pancreatic lipase activity, demonstrating their potential as lipase inhibitors. Specifically, at a concentration of 1 mg/mL, the highest inhibition rate (77%) was observed for EDm. To confirm these results, the inhibitory effect of these extracts on enzymes was tested in vivo. The oral intake of aqueous extract markedly reduced starch- and sucrose-induced hyperglycemia in healthy rats. Administration of the ethanolic extract at a specific dose of 150 mg/kg significantly reduced postprandial glycemia compared with the control group. It is, therefore, undeniable that cannabis extracts represent a promising option as a potentially effective treatment for type 2 diabetes.

Cerebral infarction is a type of ischemic stroke and is one of the main causes of irreversible brain damage. Although multiple neuroprotective agents have been investigated recently, the potential of DL-2-amino-3-phosphonopropionic acid (DL-AP3) in treating oxygen-glucose deprivation (OGD)-induced neuronal injury, has not been clarified yet. This study was aimed to explore the role of DL-AP3 in primary neuronal cell cultures. Primary neurons were divided into four groups: (1) a control group that was not treated; (2) DL-AP3 group treated with 10 μM of DL-AP3; (3) OGD group, in which neurons were cultured under OGD conditions; and (4) OGD + DL-AP3 group, in which OGD model was first established and then the cells were treated with 10 μM of DL-AP3. Neuronal viability and apoptosis were measured using Cell Counting Kit-8 and flow cytometry. Expressions of phospho-Akt1 (p-Akt1) and cytochrome c were detected using Western blot. The results showed that DL-AP3 did not affect neuronal viability and apoptosis in DL-AP3 group, nor it changed p-Akt1 and cytochrome c expression (p > 0.05). In OGD + DL-AP3 group, DL-AP3 significantly attenuated the inhibitory effects of OGD on neuronal viability (p < 0.001), and reduced OGD induced apoptosis (p < 0.01). Additionally, the down-regulation of p-Akt1 and up-regulation of cytochrome c, induced by OGD, were recovered to some extent after DL-AP3 treatment (p < 0.05 or p < 0.001). Overall, DL-AP3 could protect primary neurons from OGD-induced injury by affecting the viability and apoptosis of neurons, and by regulating the expressions of p-Akt1 and cytochrome c.

Objective: Stelechocarpus burahol, (Bl.) Hook f. &amp; Th. is a plant widely distributed in Java Island of Indonesia. The aim of this study is to identify compounds from the leaves of S. burahol that exhibited activity as xanthine oxidase inhibitor.Methods: The leaves were extracted with aqueous ethanol and hidrolized with HCl methanol, then partitioned sequentially with chloroform and ethyl acetate. The ethyl acetate fractions were separated by coloumn chromatography with cellulose as stationary phase and methanol 50% as mobile phase.Results: Purification from this extracts afforded three compounds with one compound identified, namely Kaempferol. The four compounds possessed as xanthine oxidase inhibitor with IC50 values ranging from 0.27 to 0.45 μg/ml.Conclusion:Kaempferol exhibited the highest inhibition of 0.27 μg/ml.Keywords: Kaempferol, Xanthine Oxidase Inhibitor, Stelechocarpus burahol, (Bl.) Hook f. &amp; Th.

Phorbol esters increase insulin binding in astrocytic glial but not neuronal cells in primary culture from the brain

Objective: The present study aimed at investigating the acute toxicity, the ex-vivo antispasmodic potential of aqueous and ethanolic Rumex bequaertii leaves extracts in isolated ileum fragment and antimicrobial activity of Rumex bequaertii ethanolic leaves extract in animal models infected with Salmonella typhi.&#x0D; Methods: Acute toxicity using a single dose of ethanolic extract of Rumex bequaertii at 2000 mg/kg was administered to female rats and effects were observed during 14 days. Different cumulative concentrations of the aqueous and ethanolic extracts of Rumex bequaertii leaves were tested for spontaneous contractions and potassium chloride-induced contractions in rat ileum fragment. Different doses of the ethanolic extract of Rumex bequaertii leaves were tested for antidiarrheal activity in Wistar rats infected with Salmonella typhi.&#x0D; Results: Rats given the single dose of Rumex bequaertii ethanolic extract showed no significant changes in body and organs weights compared to control rats. Administration of extracts at all tested concentrations resulted to inhibition of ileum contractions. Inhibition of spontaneous contractions of ileum fragment by aqueous extract ranging from 24.48 to 90.19 %, for 2.91, to 15.25 mg/mL, respectively, while ethanolic extract was from 42.00 to 83.72 %, for 0.99, to 5.66 mg/mL, respectively. Concerning potassium chloride-induced contraction, aqueous extract concentrations (from 2.91 to 15.25 mg/mL) inhibited contractions from 31.46 to 67.23 %; the ethanolic extract (0.99 to 5.66 mg/mL) inhibited from 38.25, to 82.60 % and verapamil from 14.31 to 80.70 %, at 0.06 to 0.34 mg/mL. After administration of ethanolic extract, all tested doses resulted to reduction of Salmonella typhi load in stools and blood, with activities being duration and dose dependent. Conclusion: The lethal dose 50 of the Rumex bequaertii ethanol extract is greater than 2000 mg/kg. Aqueous and ethanolic leaves’ extracts of Rumex bequaertii possess ex-vivo antispasmodic and ethanolic leaves extract of Rumex bequaertii possess antimicrobial activity.

Intercellular adhesion plays a role in cancer formation and protein has a key potential in maintaining cell adhesion, including syndecan-1. Meanwhile, oral cancer originates from the oral epithelium, which has an invasive and metastatic level. Its treatments involving chemotherapy and radiotherapy commonly leave unfavorable side effects, hence, suitable alternatives are needed. Natural ingredients are widely used as an alternative treatment for cancer, for example, Ganoderma lucidum (G. lucidum) which has anti-cancer and anti-angiogenic properties, induces apoptosis, stimulates an immune response, inhibits the degradation of Extracellular matrix (ECM), reduces inflammation, affects cell cycles, cytotoxic, and acts as an antioxidant.This study aims to determine the effect of ethanol extract from Ganoderma lucidum Cianjur isolate on syndecan-1 expression in KB CCL-17 oral cell cancer. This was an experimental study with a post-test only control group design, the treatment group used G. lucidum ethanol extract with a concentration of 2.12 μg/ml (P1), 4.24 μg/ml (P2), and 8.49 μg/ml (P3), while the positive control group used cisplatin with a concentration of 11.5 μg/ml (K1). In contrast, the negative control used aquadest (K0), while syndecan-1 expression was observed using the immunohistochemical examination.The highest syndecan-1 expansion rate was found in the treatment group with a concentration of 8.49 μg/ml. A significant difference was indicated by one-way ANOVA (p&lt;0.05) between K0 - K1, K0 - P1, K0 - P2, K0 - P3, K1 - P1, K1 - P2, K1 - P3, P1 - P2, as well as P1 and P3. The administration of ethanol extract from G. lucidum Cianjur isolate increases syndecan-1 expression in KB CCL-17 oral cell cancer.

Mustard green leaves (Brassica rapa L) is one of the Indonesian medicinal plants that can be used as an antidiabetic drug. This study aims to prove that administration of ethylacetate fracion of ethanol extract of mustard greens (Brassica rapa L) leaves increased superoxide dismutase (SOD)`level in hyperglycemic Wistar rats. Mustard green leaves powder (Brassica rapa L) was extracted by maceration using 96% ethanol to yield crude ethanol extract. 30 Wistar rats were divided into 5 groups consisting of 6 rats each group. Negative group, P0 (received food standart only), positive control, P1 (induced with streptozotocin and given glibenclamide drug); P2, P3, and P4 were treatment groups (induced with streptozotocin and given ethanol extract of mustard green at doses of 0.5, 2.0, and 5.0 mg/KgBw/day respectively). The dose of streptozotocin used to induce hyperglycemia in all rats was 125 mg/KgBw/day. The level of superoxide dismutase was examined before inducing streptozotocin and after the rats hyperglycemic. The ethanol extract was then fractionated into ethyl acetate fraction and then idenfified using GC-MS. The result showed that administration of ethanol extract at doses 5 mg/KgBW significannly increased SOD level(4.13 ± 1.18 ng/mL) of hyperglycemic rats as compared to negative control (2.75 ± 0.55ng/mL). Analysis GC-MS spectra of the ethylacetate fraction of ethanol extract of mustard green showed 6 major peaks assigned as vinyl propionste, buthyl formste, 2-methoxy4-vinylphrnol, 13-oxa-dispiro{5.0.5.1}trican-1-one, 1-methyl isoeugenol and 3-isopropoxy-5-methyl-phenol.

The amyloid precursor protein (APP) and its proteolytic product amyloid beta (Abeta) are associated with both familial and sporadic forms of Alzheimer disease (AD). Aberrant expression and function of microRNAs has been observed in AD. Here, we show that in rat hippocampal neurons cultured in vitro, the down-regulation of Argonaute-2, a key component of the RNA-induced silencing complex, produced an increase in APP levels. Using site-directed mutagenesis, a microRNA responsive element (RE) for miR-101 was identified in the 3'-untranslated region (UTR) of APP. The inhibition of endogenous miR-101 increased APP levels, whereas lentiviral-mediated miR-101 overexpression significantly reduced APP and Abeta load in hippocampal neurons. In addition, miR-101 contributed to the regulation of APP in response to the proinflammatory cytokine interleukin-1beta (IL-lbeta). Thus, miR-101 is a negative regulator of APP expression and affects the accumulation of Abeta, suggesting a possible role for miR-101 in neuropathological conditions.

Arcangelisia flava, with its secondary metabolites in flavonoids, has shown promising potential as an alternative treatment for hyperuricemia. The study aimed to determine the effectiveness of the ethyl acetate fraction from the ethanolic stem extract of Arcangelisia flava in inhibiting xanthine oxidase. This research, conducted at the Medical Basic Chemistry Laboratory Universitas Sriwijaya in September–December 2022, used in vitro study methods. The stem of Arcangelisia flava was extracted by maceration using ethanol. The ethanolic stem extract of Arcangelisia flava was then fractionated using n-hexane and ethyl acetate. The ethyl acetate fraction and ethanolic extract of Arcangelisia flava were measured to inhibit the xanthine oxidase using UV-vis spectrophotometry with allopurinol as a comparison. The IC50 was calculated by linear regression. The ethanol extract and ethyl acetate fraction have flavonoids, alkaloids, terpenoids, and quinones. The IC50 value of the ethanol extract was 30.04 ppm, the ethyl acetate fraction was 23.99 ppm, and the allopurinol was 17.16 ppm. The ethyl acetate fraction inhibited xanthine oxidase better than the ethanol extract. The study's significant finding is that the ethyl acetate fraction of the ethanolic stem extract of Arcangelisia flava strongly inhibits xanthine oxidase, offering a potential new avenue for treating hyperuricemia.