Abstract
Studies are reported on release of triglycerides during perfusion of livers of male Sprague-Dawley rats fed a fat-free diet or diets containing hydrogenated coconut oil or corn oil. Perfusions were carried out with Krebs-Ringer bicarbonate buffer containing albumin with and without infusion of oleate or linoleate. Infusion with sodium oleate or linoleate caused an accumulation of triglycerides in the livers of the corn oil-fed animals and stimulated the release of triglycerides into the perfusing medium. In similar experiments with essential fatty acid-deficient animals, which were fed fat-free diets or diets containing hydrogenated coconut oil, there was no increase in secretion of triglycerides into the perfusate, and the amount of triglyceride which accumulated in the liver was greater than in the livers of the control (corn oil-fed) animals. Tracer experiments with oleate-1-(14)C or linoleate-1-(14)C also showed that with livers of essential fatty acid-deficient animals, secretion of triglyceride into the perfusate was not stimulated by infusion of fatty acids into the perfusing medium. It is concluded that impairment of the secretion of triglycerides is a factor in the accumulation of fat in the livers of essential fatty acid-deficient animals.
Highlights
Infusion with sodium oleate or linoleate caused an accumulation of triglycerides in the livers of the corn oil-fed animals and stimulated the release of triglycerides into the perfusing medium
Sinclair and Collins (2) reported that there was no impairment of T G secretion from the livers of essential fatty acid(s) (EFA)-deficient animals on the basis of the experiments with Triton injection, and they concluded that the etiology of the fatty liver in EFA deficiency was different from that in experimentally induced fatty liver
Designate the number of animals used). These results showed that the effect of linoleate was essentially the same as that of oleate and that impairment in the secretion of T G in groups I and 11, in which oleate was infused into the perfusate, was not due to suppression of the utilization of oleate
Summary
Infusion with sodium oleate or linoleate caused an accumulation of triglycerides in the livers of the corn oil-fed animals and stimulated the release of triglycerides into the perfusing medium. Results of the Triton experiments upon which their conclusions were based, appeared to be inconsistent with observations of low levels of plasnia VLDL and T G observed in animals with an EFA deficiency. Because this point is basic to the mechanism of the accumulation of fat in the liver of EFA-deficient animals, it was decided to investigate further the release of triglyceride via isolated liver perfusion experiments.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
