Abstract
The short-term insemination technique has been widely applied, although it is not clear whether the mechanical desorption of cumulus cells can affect normal fertilization and birth outcomes. Therefore, the present study aimed to evaluate whether early cumulus cell removal could affect in vitro fertilization outcomes. Based on a history of infertility and semen analysis, 61 patients were identified, and the cumulus cells were mechanically removed at either6 h post-insemination (Group A, 387 mature oocytes) or 18 - 20 h post-insemination as traditional in vitro fertilization (Group B, 424 mature oocytes). There were no significant differences in the rates of fertilization, available embryos, high quality embryos, birth, premature birth, pregnancy, or implantation when we compared the two groups. However, Group A exhibited a significantly higher polyspermy rate, and significantly lower rates of blastocyst formation and high-quality blastocyst formation, compared with Group B. Therefore, the results of present study indicate that early cumulus cell removal (at 6 h post-insemination) may negatively affect the rates of polyspermy, blastocyst formation, and high-quality blastocyst formation.
Highlights
The short-term insemination technique has been widely and successfully applied, and it is gradually replacingHow to cite this paper: Liu, J.L., Chen, M.Y., Lin, C.L., Weng, X.J., Meng, Z.M. and Tang, W.J. (2015) Effect of Early Cumulus Cell Removal on the Fertilization and Clinical Outcome in Human in Vitro Fertilization
Among the 61 patients, 5 patients were excluded after receiving early rescue intra-cytoplasmic sperm injection (ICSI) after 9 h of co-incubation, due to a low fertilization rate or total fertilization failure
811 mature oocytes were collected from remaining the patients, and the oocytes were randomly divided into two groups: Group A (387 mature oocytes) underwent early cumulus cell removal at 6 h post-insemination, and the Group B (424 mature oocytes) oocytes were transferred to new sperm-free insemination medium after 6 h of co-incubation, and the cumulus cellswere removed at 18 - 20 h post-insemination
Summary
The short-term insemination technique has been widely and successfully applied, and it is gradually replacingHow to cite this paper: Liu, J.L., Chen, M.Y., Lin, C.L., Weng, X.J., Meng, Z.M. and Tang, W.J. (2015) Effect of Early Cumulus Cell Removal on the Fertilization and Clinical Outcome in Human in Vitro Fertilization. The short co-incubation allows embryologists to observe whether oocyte activation has occurred, and to immediately remedy failed activation. It is unclear whether the mechanical desorption of cumulus cells can affect normal fertilization or embryonic development potential. Various studies have demonstrated that early cumulus cells removal could affect fertilization and embryonic development potential, contradictory findings are reported [2]-[5]. Their outcome analysis only examined fertilization outcomes, and did not provide data regarding birth outcomes. The effects of early cumulus cell removal remain controversial
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