Effect of Distreptaza Distrept on the specific activity of fluconazole against Candida biofilms

Abstract

The ability of yeast-like fungi to form biofilms (structurally organized microbial communities) leads to chronicity of the inflammatory process and recurrent course of mycoses. Biofilms formed by the fungus Candida spp. are characterized by increased resistance to most antifungals and effectors of the immune system. Promising antibiotics are enzymes. Their specificity to the structural components of the biofilm can be used to prevent biofilm formation and destruction of the formed biofilm. The article presents the results of studying the sensitivity biofilms formed by Candida fungi to the action of the enzyme drug Distreptaza Distrept, as well as its effect on the specific antifungal activity of fluconazole.Research objective: to evaluate the Distreptaza Distrept impact on the antifungal activity of fluconazole on biofilms of Candida spp.Materials and methods. One-day cultures of C. albicans and C. glabrata were used in the experiments. To study the effect on biofilm formation Distreptaza Distrept, fluconazole solutions and microorganisms were applied simultaneously, when exposed to the formed biofilms for 24 h after application of the fungal inoculum. The experiments were carried out in accordance with conventional methods based on the sorption of gentian violet by biofilm structures with subsequent desorption of the dye in an organic solvent.Results. Experiments have shown that Distreptaza Distrept disrupts the film formation of C. glabrata, the inhibition is 85.6%. The enzyme preparation affects the biofilms formed by yeast-like fungi, biomass of C. glabrata decreases by 43.6%. It was found that Distreptaza Distrept enhances the specific antibiotic action of fluconazole at the stage of film formation against C. albicans by 13.1%, C. glabrata by 70.4%. Biomass of the one-day biofilm formed by C. glabrata is reduced by 80.2% if there was an enzyme drug and fluconazole in the incubation medium.Conclusion. Distreptaza Distrept has antibiotic biofilm activity, increases the specific antifungal effect of fluconazole on biofilms of Candida fungi. The data obtained indicate the feasibility of this drug in recurrent infections caused by yeast-like fungi, including diseases caused by azole-resistant strains of Candida spp.