Abstract
The work presented here is the first to examine the impact of Lactobacillus rhamnosus GG ATCC 53103 and Saccharomyces boulardii metabolites obtained using the author`s method on the formation of biofilm forms of bacteria. The structural components of the probiotic microorganisms were obtained using the method of physical disintegration – low frequency ultrasound waves produced by a G3-109 generator. Metabolites were obtained by cultivating L. rhamnosus and S. boulardii in ultrasound disintegrates of lactobacteria and Saccharomycetes. The impact of biologically active substances on the formation of biofilm of Corynebacterium ulcerans tox+ 112, C. diphtheriae gravis tox+ 108, by antibiotic-resistant Pseudomonas aeruginosa PR, Klebsiella pneumoniae PR, Lelliottia amnigena (Enterobacter amnigenus) PR and P. aeruginosa AТСС 27853 reference strain was studied using the spectrophotometric method. For the first time, we proved that L. rhamnosus GG and S. boulardii metabolites and combinations of metabolites of Saccharomycetes and lactobacteria, obtained by cultivating primary producers in their disintegrates, damage preformed 24-hour biofilms of gram-positive and gram-negative bacteria. The representatives of Corynebacterium exhibited higher sensitivity to the filtrates of disintegrates and products of vital activity of lactobacteria and Saccharomycetes than gram-negative pathogens. High parameters of decrease in optical density of preformed biofilms of Corynebacterium and antibiotic-resistant gram-negative bacteria were observed under the influence of combination of L. rhamnosus GG and S. boulardii metabolites (by 1.3–2.6 times). However, the largest reduction of the optical density of the formed biofilm of all studied strains was observed under the influence of metabolites of lactobacteria (by 1.5–5.3 times). Biologically active substances of L. rhamnosus GG and S. boulardii obtained using the author’s method can be used as candidate preparations which could have a strong influence on the process of the formation of the biofilms and preformed biofilms, and also as a preparations of substitution/addition of therapeutic prescription.
Highlights
IntroductionThanks to the scientific achievements of recent years, there has been great progress in understanding the complexity of the process of formation of biofilms, their importance in the development of infectious diseases of different genesis, etiological role in development of chronic diseases, and the difficulty of eliminating biofilm forms of pathogens as a result of their high resistance to antimicrobial preparations (Rybalchenko et al, 2010; Zhejun et al, 2017; Wang et al, 2018)
Thanks to the scientific achievements of recent years, there has been great progress in understanding the complexity of the process of formation of biofilms, their importance in the development of infectious diseases of different genesis, etiological role in development of chronic diseases, and the difficulty of eliminating biofilm forms of pathogens as a result of their high resistance to antimicrobial preparations (Rybalchenko et al, 2010; Zhejun et al, 2017; Wang et al, 2018).Biologically active components and products of peptide origin, obtained from probiotic microorganisms, have demonstrated good results due to their high antimicrobial properties (Ołdak & Zielińska, 2017)
The difference between all experimental samples in relation to the control samples was statistically significant (P < 0.05): the lowest reduction of the optical density of the formed biofilm was following the action of the filtrate of fungal disintegrate, and the highest damage was observed following the action of filtrate of disintegrate of lactobacteria
Summary
Thanks to the scientific achievements of recent years, there has been great progress in understanding the complexity of the process of formation of biofilms, their importance in the development of infectious diseases of different genesis, etiological role in development of chronic diseases, and the difficulty of eliminating biofilm forms of pathogens as a result of their high resistance to antimicrobial preparations (Rybalchenko et al, 2010; Zhejun et al, 2017; Wang et al, 2018). Active components and products of peptide origin, obtained from probiotic microorganisms, have demonstrated good results due to their high antimicrobial properties (Ołdak & Zielińska, 2017). Notable antibacterial effect against gram-positive bacteria, mostly in relation to Staphylococcus aureus, was demonstrated by LHG2 bacteriocin extracted from Lactobacillus casei. Antimicrobial substances extracted from probiotic microorganisms are promising for developing new antimicrobial preparations due to their inhibiting activity against polyresistant strains, broad range of effect, fast elimination of pathogenic bioobjects, immune-modeling effect, and low speed of induction of bacterial resistance to them (Di Luca et al, 2014; Tkachenko, 2017)
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