Abstract
Centella asiatica also known as Gotu Kola is an important medicinal plant which contains several bioactive compounds such as triterpenoid saponins including asiaticoside, madecassoside, centelloside, asiatic acid, etc. In addition, Centella sp. contains other components including flavonoids, phytosterols, tannins, amino acids, sugars, etc. Due to its medicinal importance, this plant is being overexploited and it is essential to conserve this plant. In the present investigation, comparative study of different accessions of Centella asiatica for shoot multiplication in different media and growth hormones was performed. Shoot multiplication and phytocompounds production from different accessions is required to be assessed to choose an accession giving optimum production of phytocompounds. For this, different media containing different combinations of macro and micro nutrients have to be tested that influence the growth of the plant. Assessment of best culture media and concentrations of plant hormone for shoot culture are some of the critical culture conditions to achieve. So there is a need to optimise such conditions that will enhance the growth of the plant. For shoot culture, explant of different accession of Centella asiatica were inoculated in different media i.e. Murashige and Skoog (MS), Gamborg’s B5 and Nitsch medium which were supplemented with standard concentrations of plant growth hormones. The cultures were incubated at 25 ± 2°C with photoperiod of 16 hours. After six week of incubation period, highest growth was found in MS media in all accessions. Further MS medium was supplemented with different combinations of growth hormones. After six week of incubation, MS medium supplemented with 1mg/l BAP showed the highest growth of the plant followed by 2mg/l BAP+0.5 NAA.
Highlights
Medicinal plants are the traditional source of many pharmaceutically important compounds
To the best of our knowledge there is no study which describes the effect of different media on the shoot multiplication of this plant and it provides an opportunity to explore the role of media and growth hormone on the enhancement of in vitro culture of different accessions of C. asiatica
This study was an attempt to correlate the effect of different media and plant hormone concentration on the shoot multiplication of Centella asiatica accessions
Summary
Medicinal plants are the traditional source of many pharmaceutically important compounds. Centella asiatica is one of the important traditional medicinal plant belonging to family Apiaceae and commonly known as Gotu kola in India. Leaves contains high amount of triterpenoids [2] It possesses several important properties like antileprotic, anti-stress, anti-feedent, anti-tuberculosis activities, wound-healing properties [3,4], antibacterial, antherosclerosis and fungicidal activity [5]. It is used in the treatment of leprosy, wound, cancer, fever, allergies [6], abscesses, asthma, catarrh, convulsions, dysentery, eczema, gonorrhea, hypertension, bronchitis, headache, jaundice, pleuritis, rheumatism, ulcers, spasms, tuberculosis, urethritis, etc. Effects of different media like MS, Gamborg’s B5 and Nitsch and the concentrations of plant growth hormones on the growth of three Centella asiatica accessions were observed and reported
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