Abstract

Effect of different media additives on in vitro culture of three potato varieties viz.Lalpakri, Cardinal and BARI TPS-1 was investigated using single nodes as explant. The single nodes were collected from microplants of tuber sprouts that was preincubated under dark. Survivability of the cultures was statistically insignificant in respect of the media used, but the highest percentage of cultures survived on MS medium supplimented with 60 mg L-1 inositol. The time taken to initiate new shoots was minimum on MS medium supplemented with 1 mg L-1 Benzyle Amino Purine (BAP). The number of shoot was maximum on a medium containing MS + 0.25 mg L-1 Gibberellic Acid (GA3) and 2 mg L-1 Calcium Pantothenate. The maximum number of roots was obtained on medium containing MS + 0.03 mg L-1 Naphthalene Acetic Acid (NAA). Among the varieties tested, BARI TPS-1 performed better in respect of single node culture. The interaction between different potato varieties and media compositions was significant for different characters of in vitro grown plantlets.

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