Effect of Different Light Wavelengths on Zymoseptoria tritici Development and Leaf Colonization in Bread Wheat

Abstract

The wheat pathogen Zymoseptoria tritici can respond to light by modulating its gene expression. Because several virulence-related genes are differentially expressed in response to light, different wavelengths could have a crucial role in the Z. tritici–wheat interaction. To explore this opportunity, the aim of this study was to analyze the effect of blue (470 nm), red (627 nm), blue–red, and white light on the in vitro and in planta development of Z. tritici. The morphology (mycelium appearance, color) and phenotypic (mycelium growth) characteristics of a Z. tritici strain were evaluated after 14 days under the different light conditions in two independent experiments. In addition, bread wheat plants were artificially inoculated with Z. tritici and grown for 35 days under the same light treatments. The disease incidence, severity, and fungal DNA were analyzed in a single experiment. Statistical differences were determined by using an ANOVA. The obtained results showed that the different light wavelengths induced specific morphological changes in mycelial growth. The blue light significantly reduced colony growth, while the dark and red light favored fungal development (p < 0.05). The light quality also influenced host colonization, whereby the white and red light had stimulating and repressing effects, respectively (p < 0.05). This precursory study demonstrated the influence of light on Z. tritici colonization in bread wheat.