Abstract
The goal of this study was to use plant tissue culture technique in vegetative propagation of Gardenia (Gardenia jasminoides) by using single nodes and shoot tips excised from soft cuttings and treated with different concentrations of growth regulators. The results revealed that the use of different disinfistants was highly effective in reducing cultures contamination. The use of mercuric chloride (0.1%, HgCl2) for 10 minutes was very effective in preventing contamination and gave the highest survival percentage (99%). Results at initiation stage revealed that the culture of single nodes of Gardenia on a medium containing 3 mgl-1 BA+ 0.9 mgl-1 IAA gave the best growth response in which highest number of shoots and leaves. At vegetative multiplication stage, the results showed that the medium supplemented by 1.5 and 4.5 mgl-1 BA+ 0.6 mgl-1 IAA gave the highest value of growth length and gave the highest number of shoots per explant. In order to promote the shoots produced at vegetative multiplication stage to root, it was noticed that the treatment of 4 mgl-1 NAA and8 mgl-1 IAA gave the highest percentage of rooting and values of the number and length of roots. Plantlets obtained were transferred to pots and acclimatized with 95 % success.
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