Abstract
Soluble RNA polymerase enzyme was prepared from liver nuclei of male rats allowed an 18% and 6% casein diet ad libitum or fed by stomach tube with synthetic amino acid mixtures. The amino acid mixtures were either nutritionally complete or devoid of leucine or valine. It was found that the response of the Mn ++-dependent soluble RNA polymerase was related inversely to the dietary protein level (6% and 18%). After feeding diets devoid of either leucine or valine for 3 days, RNA polymerase activity was significantly decreased. These results suggest that dietary amino acid supply rapidly affects RNA polymerase activity. Food intake and fasting were shown to alter the activity of the enzyme. The enzyme activity exhibited 2 peaks during the time interval that food was allowed and the maximum increase occurred several hours after food ingestion. During the fasting period the enzyme activity decreased at each time interval the rats were killed. These data indicate that the enzyme activity is both time-dependent and contingent upon food intake.
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