Abstract
Objective To investigate the effects of decreased 5’-activated protein kinase (AMPK) activity in mouse mammary epithelium on the proliferation of ductal epithelial cells and the development of teminal end bud (TEB) in adolescent mice. Methods AMPK DN transgenic mice were successfully constructed. The fourth pair of mammary gland tissues from the right abdomen of 10 WT and DN mice were stained by whole tissue staining and PCNA immunohistochemical staining. The duct coverage area, initial duct length, number of ductal branches per millimeter, percentage of ductal invasion length, terminal tip of mammary gland tissues of WT and DN mice were analyzed. TEB average quantity, average maximum area of TEB. The ratio of PCNA positive cells to the total number of glandular epithelial cells was calculated. Results At 5th week, the number of ductal branches per millimeter in DN mice and WT mice was (1.39±0.18) mm vs. (1.21±0.11) mm, the average number of TEB was (13.99±3.14) vs. (10.65±2.09) and the average maximum area of TEB was (0.104±0.014) mm2 vs. (0.082±0.013) mm2, respectively, with significant difference (t=2.698, 2.800, 3.642, P<0.05); at 6th week, the number of ductal branches per millimeter in DN mice and WT mice was (1.59±0.013) mm2, respectively. 31) vs. (1.41±0.34) mm, the average number of TEB (15.86±3.26) vs. (12.47±2.34), the average maximum area of TEB (0.118±0.017) mm2vs. (0.087±0.014) mm2, the difference was statistically significant (t=2.406, 2.671, 4.451, P<0.05). The percentage of PCNA positive cells in mammary epithelial cells of 5 w DN mice (86.47±4.67) was significantly higher than that of WT mice (65.32±3.87)%, and 6 w DN mice (65.32±3.87)%. The percentage of cells in WT group was significantly higher than that in WT group (85.37±4.35) (63.47±3.45)%. The difference was statistically significant (t=4.614, 12.474, P< 0.05). Conclusion The decrease of AMPK activity in mouse mammary epithelium can promote the proliferation of ductal epithelial cells and the development of TEBs in adolescent mice. Key words: Mouse mammary epithelial cells; Adolescence; 5’-activated protein kinase; Proliferation; Teminal end bud
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