Abstract
Human neutrophils aggregate after exposure to the bivalent cation ionophore A23187, the synthetic chemotactic tripeptide formyl-methionyl-leucyl-phenylalanine, and the chemotactic fragment of human C5. Cells preincubated with cytochalasin B (CB) (0.5 μg/ml) showed an enhanced aggregation response to each of these three agents. By itself, CB did not aggregate the cells. The enhancing effect of CB on the aggregation response developed immediately after exposure to the drug and was readily reversible. Since A23187 and chemotactic factors stimulate the transmembrane flow of Ca 2+, Na +, and K + in the neutrophil, and since CB enhances this effect, CB may influence neutrophil aggregation through its influence on transmembrane cationic movements. It was also found that the enhancing effect of CB on neutrophil aggregation was abrogated by high (i.e., greater than 1.7 μg/ml) concentrations of the drug or by adding it to the neutrophils after the cells were exposed to a chemotactic factor.
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