Abstract
Summary A locally isolated strain of Azotobacter chroococcum was found to produce high amounts of plant-growth regulators when cultivated in a medium supplemented with combined nitrogen. In particular, ammonium sulphate and ammonium chloride were favourable for gibberellic acid and indole-3-acetic acid formation, respectively. In ammonium-enriched medium, production of the two growth-regulating substances increased by the addition of pyruvate, α-ketoglutarate and ascorbic acid. Molybdenum was required for the formation of high amount of indole-3-acetic acid, while molybdenum, iron (Fe ++ ) plus boron were favourable for active secretion of gibberellic acid. The relationship between the formation of plant-growth regulators and the sucrose concentration was studied in ammonium-enriched medium supplemented with pyruvate, α-ketoglutarate, ascorbic acid and the favourable trace elements. In case of indole-3-acetic acicl, 3% sucrose concentration was more efficient than higher concentrations. For gibberellic acid, 4% concentration was the best.
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