Abstract
The atomic force microscope (AFM) is broadly used to study the morphology of cells. The morphological characteristics and differences of the cell membrane between normal human astrocytes and glial tumor cells are not well explored. Following treatment with cold atmospheric plasma, evaluation of the selective effect of plasma on cell viability of tumor cells is poorly understood and requires further evaluation. Using AFM we imaged morphology of glial cells before and after cold atmospheric plasma treatment. To look more closely at the effect of plasma on cell membrane, high resolution imaging was used. We report the differences between normal human astrocytes and human glioblastoma cells by considering the membrane surface details. Our data, obtained for the first time on these cells using atomic force microscopy, argue for an architectural feature on the cell membrane, i.e. brush layers, different in normal human astrocytes as compared to glioblastoma cells. The brush layer disappears from the cell membrane surface of normal E6/E7 cells and is maintained in the glioblastoma U87 cells after plasma treatment.
Highlights
Plasma is an ionized gas that is typically generated in high-temperature laboratory conditions
The results of the MTT assay showed that after 72 h, around 80% of U87 cells died at 30s and 60s of plasma treatment, while E6/E7 cells remained 90% and 60% cell viability at 30s and 60s plasma treatment at 72 h post-treatment time point
atomic force microscope (AFM) images were acquired in contact mode either as topographies that show the height of contours or as deflection signal images that highlight the fine features of surface morphology
Summary
Plasma is an ionized gas that is typically generated in high-temperature laboratory conditions. The reactive ionized species, such as OH, H2O2, N2+, NO and O2-are the main components of the cold plasma jet that provides for therapeutic effects, with cancer, and with biological disinfection [5], viral destruction [6] and wound healing [7]. It is well-known that NO is an omnipresent intercellular messenger in all vertebrates, modulating blood flow, thrombosis, neuronal activity, immune.
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