Abstract
Biomethane production potential was evaluated by anaerobic co-digestion of grass silage (GS) and chicken litter (CL) in batch experiments. The aim of this study was to identify optimum enzyme treatment and co-digestion ratio for these substrates. The study is divided into trial A and trial B for enzyme treatment and co-digestion respectively. Mixed substrates were utilised in both trials which were maintained for a period of 30 days in 125 mL serum bottles at 37 °C. Preliminary batch assays in trial A for biomethane potential determination were performed using a co-digestion ratio of 2:1 (GS:CL) treated with five different concentrations of enzyme (w/v)—0.05, 0.1, 0.25, 0.5 and 1%. The highest specific methane yield (SMY) of 61 L CH4/kg was observed for 0.1% (w/v) enzyme treatment. Trial B assays were conducted for nine different GS:CL ratios—0:1, 1:0, 1:1, 1:2, 1:3, 2:3, 3:2, 3:1, and 2:1 respectively. Highest SMY were recorded for the ratios 1:0, 1:1 and 1:3 of GS:CL which correspond to 124 L CH4/kg, 137 L CH4/kg and 135 L CH4/kg respectively.
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