Effect of chiral additive on formation of cellulose triacetate chiral stationary phase in HPLC

Abstract

Cellulose triacetate, an chiral polymer that has been widely used in chiral recognition in high performance liquid chromatography, and also used in membrane science such as reverse osmosis, microfiltrtion and ultrafiltration, was coated on silica gel as stationary phase for HPLC and the pores of membrane were created by chiral additive (+)-di-O,O′- p-toluyl- d-tartarric acid, (+)-1-phenyl-1,2-ethanediol and (+)- l-mandelic acid, respectively. The results show that those stationary phases not only can separate extensively racemates, but also can resolve the chiral additive. This is a report, for the first time, that the chiral additive was used in formation of chiral stationary phase of optically active polymer. This work indicates that some optically active polymers, which have been used for chiral resolution, could soon become very attractive as new chiral stationary phase having a predictable chiral selectivity advantage.