Abstract
Objective: The aim of this work was to investigate the effect of different cytidine-5′-diphosphocholine (CDP-choline) concentrations (0.1–1 mM) on acetylcholinesterase (AChE), (Na +,K +)-ATPase and Mg 2+-ATPase activities in homogenates of adult rat whole brain and in pure (nonmembrane bound) enzymes. Design and Methods: Tissues were homogenized, centrifuged at 1000 ×g for 10 min, and in the supernatant AChE activity and Na +,K +-ATPase, Mg 2+-ATPase activities were determined according to Ellman’s method and Bowler’s and Tirri’s, respectively. Results: After a 1–3 h CDP-choline preincubation, this substance induced a maximal stimulation of 20%–25% (p < 0.001) for AChE and 50-55% ( p < 0.001) for Na +,K +-ATPase, but it did not influence Mg 2+-ATPase activity. One mM acetylcholine (ACh) resulted in an approximately 18% ( p < 0.001) AChE inhibition by excess substrate in the brain homogenate, while 0.01 mM noradrenaline did not influence Na +,K +-ATPase activity. Conclusions: CDP-choline can stimulate brain AChE and Na +,K +-ATPase independently of ACh and noradrenaline. This enzymatic stimulation may be due to the transformation of CDP-choline to membrane phophatidylcholine. The above data could explain in part the clinical effects of this substance in some neuronal disturbances.
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