Abstract
STOLL1 was the first to show that Haemonchus contortus can develop to the fourth stage without an exogenous source of nutrients. In Ringer and Tyrode solutions, about 10 per cent of exsheathed third-stage larvae attained the fourth stage within 4 days. The process was accelerated by the addition of aqueous extracts of liver, by the use of sealed, rather than unsealed, tubes and by the presence of large rather than small numbers of larvae. Under these conditions up to 50 per cent of exsheathed third-stage larvae underwent development to the fourth stage within 4 days. Silverman, Poynter and Podger2 have used a similar method to collect late third-stage and early fourth-stage larvae of several species for the preparation of antigens.
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