Effect of Calcium on Spontaneous Quantal Transmitter Secretion from ACh-Loaded Myocytes

Abstract

Spontaneous secretions occur in both neurons and non-neuronal cells, and calcium is important for these secretion processes. However, the detailed roles of calcium on the secretions have not yet been identified. In the present study, cultured Xenopus myocytes loaded with exogenous acetylcholine (ACh) into the cytoplasm in the absence of extracellular Ca 2+ undergo spontaneous quantal ACh secretion as detected by the appearance of pulsatile miniature endplate currents. Analysis of the frequencies, amplitudes, and time courses of these currents suggests that similar cellular mechanisms are involved in the secretions of ACh in normal medium and Ca 2+-free solution. Various doses of ryanodine were used to regulate the intracellular Ca 2+ to different levels. The spontaneous ACh secretion from myocytes in Ca 2+-free medium was decreased by reducing intracellular Ca 2+ levels and enhanced by increasing cytosolic Ca 2+ levels. These observations demonstrate that the spontaneous secretion from isolated myocytes and the effect of ryanodine on ACh-loaded cells are both independent of extracellular Ca 2+ while Ca 2+ in the sarcoplasmic reticulum plays a crucial role in the secretions.