Effect of burnt tire-ash (water-soluble fraction) on blood and histopathological markers in Clarias gariepinus

Abstract

Aquatic habitats serve as pollutant reservoirs and experience massive pollution disasters due to anthropogenic pressure. The effect of the Water-Soluble Fraction (WSF) of burnt tire ash (BTA) was investigated on Clarias gariepinus juvenile using blood biomarkers as well as liver and gill histology. The model fish were exposed to 5 % (0.56 g/L), 10 % (1.12 g/L) and 20 % (2.24 g/L) of 96-hr LC50 (11.2 g/L) value of BTA sub-chronic doses during the experiment. Hematological indices, the liver, and gill histology were studied throughout the 28-day exposure study, which was followed by a 14-day post-exposure trial. The studies revealed that BTA-exposed fish had reduced red blood cells, packed cell volume, and hemoglobin levels with time, whereas white blood cells and leukocyte numbers increased. Concentration-dependent histopathologic abnormalities such as hyperplasia, telangiectasis, hemorrhage, desquamation, lamellar edema, and vacuolation were observed in the gill of the affected fish, in contrast to the control. Likewise, the presence of histopathologic abnormalities in the liver of BTA-exposed fish, such as vacuolar degeneration, hyperplasia, dilated portal vein, pyknotic nucleus formations, hypertrophy hepatocytes, and aggregated macrophages, was concentration-dependent. Following apparent histologic deformities that persisted in the tissues after recovery, healing from BTA exposure was only partial. C. gariepinus is an essential bioindicator of burnt tire ash’s ecotoxicological impact. Point and non-point traces of burnt-tire ash to the aquatic systems may harm aquatic animal species, thus necessitating preventative actions.