Abstract
Study background: This in vitro study aimed to evaluate the minimum application times of three denture disinfection procedures to significantly reduce initial Candida albicans colonization. Methods: Specimens of a soft denture relining material were incubated with Candida albicans for 2.5 h and allotted to three different disinfection regimes (sodium hypochlorite 1%, effervescent cleansing tabs, and microwaving immersed in water) with application times ranging from 30 to 600 s. Adhering fungi were quantified using a bioluminescence assay in combination with an automated plate reader for cell quantification. Results: Luminescence at baseline varied between 409 and 472 relative luminescence units (rlu). A significant reduction of luminescence was found after 120 s of soaking in sodium hypochlorite (31 rlu) and effervescent cleansing tabs (35 rlu), and after 60 s of microwave irradiation (26 rlu). Conclusions: Threshold minimum application times of 120 s for sodium hypochlorite and cleansing tabs, and 60 s for microwaving were determined. A further extension of application times did not have additional antifungal effects.
Highlights
C. albicans has been considered as the most prevalent fungus in the human oral cavity and major microbiological factor in the pathogenesis of denture-related stomatitis, in which dentures may function as a reservoir of infection [1]
A great variety of cleansing agents and disinfection regimes with antifungal properties are available, but only few have proven to be effective against C. albicans [5]
A stepwise decrease of luminescence could be observed for successive microwave irradiation
Summary
C. albicans has been considered as the most prevalent fungus in the human oral cavity and major microbiological factor in the pathogenesis of denture-related stomatitis, in which dentures may function as a reservoir of infection [1]. Up to 65% of all denture wearers are affected by Candida infections [1]. Yeast cells are known to form pathogenic biofilms on artificial devices such as pacemakers, shunts, implants, and prostheses [2]. These fungal biofilms have increased resistance to host defenses and are extremely resistant to many conventional disinfectant measures [3]. A great variety of cleansing agents and disinfection regimes with antifungal properties are available, but only few have proven to be effective against C. albicans [5]
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