Abstract
Anchovy (Coilia mystus) protein hydrolysate (APH) and its Maillard reaction product (APH-M) with d-ribose were subjected to in vivo mouse behavioral trials and associated with biochemical analyses, in vitro H2O2-stressed PC12 cell assay and inhibition of acetylcholin esterase (AchE) to explore the mechanism of the behavioral trial findings. Results revealed that both APH and APH-M could alleviate undesired alterations on hippocampus ultrastructure of mice, protect PC12 from H2O2-induced oxidative stress, and inhibit AchE activity in vivo and in vitro, suggesting the well-being potential for combating memory-impairment in mice. Moreover, APH-M exhibited much stronger abilities above than APH. The test of acetylcholinergic system indicated that APH-M would improve memory mainly through regulating the AchE activity, while APH through controlling the mRNA expression of ChAT and AchE activity. Therefore, protein hydrolysates from anchovy could possess therapeutic potential for memory deficits, and Maillard reaction might further promote their memory-improving capacity.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
