Effect of amrinone on the maturation of mouse oocytes

Abstract

Objective To examine the inhibitory effect of amrinone on the maturation of mouse oocytes in vitro and in vivo, and whether it is possible to detect the potential use of the inhibitor as a potential contraceptive. Methods The effects of different concentrations of amrinone on immature mouse oocytes were observed. After oocyte maturation, intracytoplasmic sperm injection (ICSI) was carried out, and the fertilization rate, embryo formation and the comparison of the offspring were observed. The adverse reaction of mice in in vivo injection group was monitored. Results 1) With the increase of the concentration, the ammonia induced meiosis was dose-dependent. The effective concentration was 1 µmol/L in vitro, but was about 300 mg/kg in vivo. 2) Its function was reversible. After the removal of the drug, the meiosis recovered gradually and mouse oocyte matured with normal chromosome alignment and spindle organization. 3) Compared with control group, after fertilization, oocytes showed normal morphology, fertilization rate, cleavage and blastocyst formation. 4) The birth offspring showed similar body weight and fertility. 5) In vivo, a certain concentration of drug injected into mice exhibited infertility till discontinuation. Conclusion In vitro and in vivo mouse oocytes can be inhibited by amrinone and maturation is reversible, and mating experiments have proved that it may be used as a contraceptive drug. Key words: Phosphodiesterase (PDE)3 inhibitor; Amrinone; Oocytes maturation of mouse; Spindle and chromosome